المصدر: Ladislav Homolka L. Homolka (*) Department of Ecology of Microorganisms , Institute of Microbiology, Academy of Sciences of the Czech Republic , Vídeňská 1083 , Prague 4 142 20 , Czech Republic e-mail: [email protected] 2 Abstract Traditional method of the routine subculturing by transfer of fungal cultures from staled to fresh media is not a very practical means of storing large numbers of fungal cultures. It is time-consuming, prone to contamination, and does not prevent genetic and physiological changes. At present, besides freeze-drying (lyophilization), cryopreservation seems to be the best preservation technique available for fungi. Keywords Cryopreservation • Fungi collections • Liquid nitrogen • Perlite • Subculturing • Fungi storage Methods of Cryopreservation in Fungi Ladislav Homolka V.K. Gupta et al. (eds.), Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology, Fungal Biology, DOI 10.1007/978-1-4614-2356-0_2, © Springer Science+Business Media, LLC 2013