DNA Microarrays and Gene Expression From experiments Data Analysis and modeling
By
Pierre Baldi and G. Wesley Hatfield
Introduction:
Massive data acquisition technologies, such as genome sequencing high throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the m RNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role that individual genes or group of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. This interdisciplinary introduction to DNA arrays will be essential reading for researches wanting to take advantage of this powerful new technology.
Contents
- A brief history of genomics.
- DNA array formats.
- DNA array readout methods.
- Gene expression profiling experiments: problems, pitfalls, and solutions.
- Statistical analysis of array data: inferring changes.
- Statistical analysis of array data: dimensionality reduction, clustering and regulatory regions.
- The design, analysis, and interpretation of gene expression profiling experiments.
- System biology.
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