Inducing systemic resistance against Bean yellow mosaic Potyvirus using botanical extracts

A.M.M. Mahdy1; R.N. Fawzy1; M.A. Hafez1; Hanan A.N. Mohamed2

and Eman S.M. Shahwan1

 1Agic. Botany Dept., Fac. Agric., Moshtohor, 13736, Benha Univ., Egypt and 2 Agric. Genetic Engineering Res. Inst. (AGERI), Agric. Res. Center (ARC), 12619, Giza, Egypt.

Received October 2007: accepted December2007

Abstract

Mosaic with bright yellowing symptoms suggestive due to bean yellow mosaic Potyvirus (BYMV) was the most frequently found and likely most damaging virus in faba bean crop. The virus was detected in different locations in Qalyoubia Governorate using DAS-ELISA. Enhancements were introduced to Ouchterlony double diffusion test. Response of some cultivated faba bean cultivars was studied under artificial inoculation by BYMV. Delaying the sowing date, and spraying faba bean seedlings with six aqueous botanical extracts under field conditions fortnightly interval, were achieved as a simple strategy for BYMV control. Ribosome-inactivating proteins (RIPs) were demonstrated by protein profile pattern of both faba bean and botanicals using SDS-PAGE. ELISA was used as a diagnostic tool at the beginning of the study and at the end to insure no faba virus infections after 3 botanical extract sprayers.