Biological studies on Pepper mild mottle virus affecting pepper plants in Egypt
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S.A. El-Kewey. 1, M. A. S. El-Kady2, S.A. Sidaros1, A.A. Emeran1 and Samaa Abd El-Khalik3
1Agricultural Botany Department, Fac. of Agri., Kafrelsheikh Univ. 2Virus and Phytoplasm Res. Dept. Plant Pathology Res. Inst. Agricultural Research Center(ARC) . 3Plant Disease Research Section, Sakha, Kafr El-Sheikh Plant Pathology Res. Inst, ARC, Egypt
Received October 2007: accepted December2007
Abstract:
An isolate of PMMoV was obtained from naturally infected pepper plants grown in Kafr El-Sheikh Governorate, exhibiting different patterns of mottling, chlorosis with leaf distortion and stunting. The isolated virus was identified on the basis of host range, symptomatology, stability in crude extracts, modes of transmission, serological reaction, inclusion bodies and electron microscopy. Host range studies revealed that the isolated virus infected thirteen plant species and cultivars belonging to two families.The virus reacted systemically on all pepper cultivars tested (C. annum cv. california wonder, C. frutescens. cv. chilli) and N. clevelandii. The virus was inactivated in sap after heating for 10 min at 95°C but not at 90°C. The dilution end-point was between 10-7 and 10-8. Virus infectivity in vitro was maintained more than 30 days storage at room temperature (26- 28°C). The virus under study was transmitted mechanically and by infected seeds but cannot be transmitted by M. persicae and Aphis fabae. The identification of the virus was confirmed serologically by indirect ELISA using TMV specific antiserum. Positive reaction obtained indicated that the virus under study was related serologically to Tobamovirus. Light microscope examinations revealed amorphous cytoplasmic inclusions (X- bodies) in PMMoV infected cells. Electron micrograph of puified virus preparation revealed rod shaped particles of about 312 nm long and 18 nm width typical of tobamo viruses.
Key words: Pepper mild mottle virus, Tobamovirus, Transmission, Electron microscope, Inclusion bodies.
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