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Serial changes in whole blood units during storage to evaluate safety  for blood transfusion

 Mohammed Shnain Ali

College of dentistry/Karbala University.

ABSTRACT

During storage of whole blood units in blood banks, certain changes are expected like functional and biochemical changes. This work aims to evaluate safety of whole blood units for blood transfusion. In this study thirty units of whole blood were selected for study. Each unit was assessed serially at days 1, 7, 14, 21 and 28 of storage by visual assessment, plasma potassium level, plasma glucose level and plasma Hb (free Hb). The maximum acceptable percentage of hemolysis was considered to be 0.8%.

The results indicated that visual assessment of whole blood units showed evidence of pinkish discoloration of supernatant plasma in 14 out of 30 units (i.e  46.67%) at days 7, 14, 21 and 28 of storage. There was a significant rise in plasma potassium concentration (P<0.05) in 100% of units from day 1 to 28 of storage while there was a significant fall in plasma glucose level (p<0.05)  in 100% of units from day 1 to day 28 of storage. Free Hb levels were significantly elevated from day 1 to 28 of storage reaching a maximum value of 100 mg/dl (i.e  0.36% hemolysis which is much lower than the maximum percentage of acceptable hemolysis).

In conclusion, serial changes of whole blood units depending on both visual and laboratory assessment, but not on visual assessment alone, were within the acceptable limits of safety for blood transfusion.

Key words: Whole blood units, Serial changes, hemolysis.