Four fungal species (Arthrobotrys sp., Hirsutella rhossiliensis, Trichoderma harzianum, T.viride) and six bacterial isolates (Paenibacillus polymyxa, Bacillus thuringiensis, B. megaterium, B. subtilis, Serratia sp., Pseudomonas fluorescens) were selected to determine their ability to suppress the reproduction of Meloidogyne incognita on susceptible grapevine plants [Vitis venifera L. (cv. Flame Seedless)] under greenhouse conditions. The susceptible grapevine plants were treated by all previous microorganisms individually by rate 13108 CFU/20ml. The bio-agents inoculums were added to plants by two different ways. The first depends on adding the bio-agents only once (one week before nematode inoculation; 3000J2/plant) and the second adding the bio-agents twice (one week before and one week after nematode inoculation). Nematicide treatment, VYDATE_ as liquid 0.5% was used for comparison. All tested treatments (one week before nematode inoculation) significantly decreased number of J2 in the soil by rate 83%, number of root galls and number of egg-masses per plant 83.7, 85% respectively. The highest reduction was evident with Bacillus subtilis, in reducing root galling and number of eggmasses per plant. B. thuringiensis, Paenibacillus polymyxa and the fungus Arthrobotrys sp., gave the same effect in reducing root galling and number of egg-masses per plant. The least effective bio-agents in reducing nematode population were recorded in Trichoderma harzianum and B. megaterium. On the other hand when the fungi and bacteria added twice (one week before and one week after nematode inoculation), that increased the effect of bio-agents in reducing root galling and number of egg-masses per plant. B. megaterium was followed by the fungus Arthrobotrys sp., since they gave better results more than nematicide treatment (VYDATE_ 0.5%). The number of second stage juveniles in the soil was differ according to the different treatments. Fresh weights of root, shoot and their length were taken as a criteria for grapevine growth response. Plant treated by B. thuringiensis, one week before nematode inoculation gave the highest increase in the whole plant weight followed by B. subtilis. On the other hand when the bio agent were added twice, the highest increase in the whole plant was obtained in plant treated by Arthrobotrys sp., and by Hirsutella rhossiliensis, followed by B. thuringiensis.