A survey to study the presence and distribution of plant-parasitic nematodes associated with grape (Vitis vinifera L) was conducted in Ismailia vineyards. A totals of 460 plant and soil samples were collected from vineyards of five different localities in Ismailia governorate viz. “Ismailia, El-Tall al Kabir, Abou-Swair, El-Kantara Sharq, and El-Kassasen". The results obtained from the survey showed that the population varied in their frequency and distributed among surveyed vineyards. There were eleven plant parasitic nematode genera found associated with common grape plants in Ismailia Governorate. These genera were Meloidogyne spp.,  Hoplolaims spp., Tylenchorhynchus spp.,  Xiphinema spp., Pratylenchus spp., Rotylenchulus spp., Helicotyleuchus spp., Trichodorus spp.,  Criconemella spp., Tylenchulus semipenetrans and Longidorus spp. The root-knot nematode Meloidogyne spp. was the most frequently genus occurred in grape with FO of 100% in all the studied regions. In El-Tall al Kabir region the data revealed the citrus nematode, Tylenchulus semipenetrans and dagger nematode,  Xiphinema spp. were widely distributed with 40 and 65 FO% respectively from the collected samples.  The needle nematode, Longidorus spp. and the lesion nematode, Pratylenchus spp. occupied the rank of the most second distributed common genera in the third region El-Kassasen with 74 and 70% respectively. While, in fourth region Abo-Sewir, the spiral nematode, Helicotylenchus spp. and the reniform nematode, Rotylenchulus reniformis  was the most common genera with 67 and 45 F O % respectively.  In region El-Qantara Shark, the stunt nematode, Tylenchorhynchus spp. was found with FO of 70%. While, the stubby root nematode,   Trichodorus spp. was the lowest distributed genus with FO of 20%.