The present invention is depending on evaluate the phytotoxic potential of natural products of aromatic plant for weed control. Allelopathy phenomena are kinds of weed / crop, weed / weed or crop / crop interference where some chemical constituents are released by some plant species which can cause inhibitory effect on the growth of the other species. Three experiments carried out at the wire house of Weed Research Laboratory at Giza, field crops and horticulture farm experiments at Sids Research Station, Beni Suef Governorate, Agriculture Research Center, conducted during the period of 2014 to 2017 to study the exploration portion of the phytotoxic (allelopathic) potential of aqueous extracts from dry leaves, stem and seeds of ocimum basilicum on commercially important agricultural crops and its associated weeds. Results show that tomatoes crop as well as Portulaca oleraceus L, Amaranthus spp, Euphorbia helioscopia L, Ammi majus, L., Anagallis arvensis were sensitive to aqeous O. basilicum extracts when applied at the concentration 3, 4 and 5% post emergence at 2-4 leaf stage of weeds, but, aqueous O. basilicum extracts did not any effect on maize, kidney bean, as summer crops and wheat, Chickpea, clover and onion winter crops, or Beta vulgaris, L, Capsella bursa-pastoris, L, Malva parviflora, L., Sonchus oleraceus, Rumex dentatus, L., Medicago polymorpha, L, Melilotus indica L., Trifolium respinatum L., at different concentration. However, Chenopodium album, Amaranthus hybridus and Portulaca oleraceus were susceptible to low concentrations of Ocimum extract 1, 2, 3, 4 and 5% when applied post- emergence at stage 2-4 leaves of weeds. O. basilicum extracts at the concentration 3, 4 & 5% caused reduced percentage of P. oleraceus; T. portulacastrum and C. rotundus by 60, 80 & 86%; 75, 80 & 90% and 75, 90 & 91%, respectively, in average in all experiments under study. This method for weed control is characterized by preserving the environment from contamination by the herbicide that are used for reducing weed density incidence, obtaining a healthy, clean and safe agricultural product for public health, with ease of manufacturing and saving hard currency for importing herbicides
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