Allelopathic effect of Ocimum basilicum L. extracts on weeds and some crops and its possible use as new crude bio-herbicide


M. S., Mekkya; A. M. A., Hassaniena, * E. M. Kamela and A. E. A. Ismaila

a Weed Research Central Laboratory, Agricultural Research Center, Giza

9, El-Gamaa St. Giza, Egypt


Experiments were carried out during 2018 and 2019 seasons, to investigate the allelopathic potentiality of Ocimum basilicum extract as a biodegradable herbicide against broad-leaved and grassy weeds. Leaves and flowers of Ocimum into flower were used to prepare extracts by three solvents (methanol, acetone and distilled water) to assay their effects on maize and soybean seedlings and dominant weeds in pots at Giza and fields Sids Stations. The inhibitor of 50% reduction (ID50) values on germination percentage for Amaranathus was about 0.2 to 0.9 mg, whereas for maize and soybean were 10 to 30 and 17 to 23 mg fresh weight of Ocimum equivalent extracts ml-1 respectively. Pots experiments showed that Amaranthus and Portulaca weeds were affected by extracts when applied post emergence which gave 33-68% reduction in fresh weight of Amaranthus/Portulaca mixture by the three studied extracts. Field experiment at Sids Station showed that the effect of different formulations, crude extract by acetone alone and extract plus camphor oil were more effective against broadleaved and grassy weeds than using its formulation with adjuvant where about 40.48 kg fresh weight of Ocimum equivalent extracts ml-1/hectare when applied post emergence gave 97 to 99% control of weed biomass, which equal the recommended herbicide (glufosinate ammonium) effect. The chemical compositions of extracts were analyzed by GC–MS to determine chemical compositons of the developing new bio-herbicide. Thus, the present results suggest clearly that farmers can use easily extract 40.48 kg/hectare of Ocimum to control weeds in horticulture orchard fields during summer season.


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نشرت فى 21 ديسمبر 2019 بواسطة mohammedmekky

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