ROLE OF PHYTASE SUPPLEMENTATION INTO MUSCOVY DUCKS DIET IN THERMO - AND OSMOREGULATION DURING SUMMER SEASON

A. S. O. EI-Badry; M. H. Mahrousa*; M. H. Fatouh and  A. S. Abd EI-Hakim**

Department of Rabbit; Turkey and Water Fowl Breeding Research Animal Production Research Institute. Ministry of Agriculture. Dokki. Giza. Egypt

* Department of Cell Biology. National Research Center. Ministry of Scientific Research, Dokki, Giza. Egypt

**Poult. Nutr. Dep., Animal Production Research Institute. Ministry of Agriculture. Dokki. Giza. Egypt

Abstract: A total of 126 Muscovy ducklings one day old were used in the present study to assess the effect of phytase supplementation on HSP70 gene expression, thermal reaction, plasma osmotic pressure, hematological parameters, some plasma parameters. liver and kidney functions during summer conditions. Ducklings were divided into six equal groups. Three diets were formulated to contain 0.25, 0.34 and 0.45% NPP for feeding from I d to 3weeks of age, and 0.21. 0., 30 and 0.40 % NPP for feeding from 3 to 11 weeks of age. respectively. Phytase enzyme was added to all diets at two levels (0 and 750 U\Kg).

Results showed that HSP70 and respiration rate increased significantly with increasing dietary NPP level. Enzyme addition into diets caused a significantly increase in HSP70 compared with other treatments while, body temperature did not differ significantly by dietary NPP level without or with enzyme addition. With respect to liver (as measured by AST and ALT) or kidney functions (as measured by uric acid and creatinine levels), ducks fed diet contained lower NPP recorded higher levels of these plasma components than the other groups. While, addition of phytase to ducks diet had no deleterious effect on liver or kidney function. Plasma osmotic pressure significantly decreased with increasing NPP level and phytase supplementation.

Neither phosphorus levels nor phytase inclusion had significant effect on WBC, RBC and PCV. While, hemoglobin concentration increased significantly with increasing dietary NPP level. Enzyme addition into diets caused a significant increase in hemoglohin concentration (Hb) compared with other treatments.

Present data indicates that phytase supplementation had some beneficial effects on some physiological, hematological parameters and plasma osmotic pressure in Muscovy ducks during summer conditions.