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Protective role of olive oil and /or folic acid on some biochemical biomarkers related to cardiac disease in Rabbits exposed to Methionine overload

Khalisa Khadim Khudiar1 and Wefak Gaborry Al- Bazii2

1-      Department of Physiology and Pharmacology,College of Veterinary Medicine, Baghdad Univ.

2-      Department of Environmental Health, College of Applied Medical Science,

University of Karbala, Iraq

ABSTRACT

            The present study was aimed to investigate the protective role of olive oil and/or folic acid against cardiac damage induced by methionine overload rabbits. Forty male rabbits were divided into five groups (8/group), the first group was intubated with tap water and served as control group(GI). Rabbits in the second group were intubated orally with 100 mg/kg B .W of methionine (GII). While animals of the third group were intubated with methionine and 0.51 ml/kg B.W. of olive oil (GIII), in addition to methionine, rabbits in the fourth group (GIV) were intubated orally with 0.07 mg/kg B.W of folic acid . Animals of the last group (GV) were intubated with methionine, folic acid and olive oil. Fasting blood samples were collected at zero,28 and42 days of experiment for measuring : serum total cholesterol(TC),triacylglycerol (TAG), low density lipoprotein(LDL-C ),very low density lipoprotein(VLDL-C), high density lipoprotein (HDL-C), glutathione (GSH) and Malondialdehyde (MDA) concentrations . Furthermore, aortic and cardiac muscle tissue were assessed for histopathological changes . The results revealed that intubation of male rabbits with 100mg/kg B.W .of methionine daily  for four weeks caused a significant (p<0.01)  increase in serum TC , TAG, LDL-C, VLDL-C and MDA concentration,   and a significant decrease (p<0.01) in serum   GSH and HDL-C concentration,  comparing to control ,folic acid and olive oil  treated groups. Histological section revealed that methionine initiated aortic atheromatus lesion characterized by a thickness of the intimae, infiltration of inflammatory cells with focal foamy cells in subintimal layer and infiltration of inflammatory cells in cardiac muscles.  The result also showed that oral olive oil and /or folic acid caused significant correction  of the previous parameters manifested by significant elevation in serum HDL-C , GSH,  in addition to significant suppression of serum TC , TAG , LDL-C ,VLDL-C ,MDA concentrations ,as well as complete regression of atheromatous lesion  were observed after olive oil intubation.  In conclusion, the results of this study confirm the protective role of olive oil against cardiac damage (both structurally and functionally ) induced by methionine overload and documented the prevalence of olive oil upon folic acid in all measures issued.

Key word: Olive oil, Folic acid, Lipid profile, Atherosclerosis, MDA.