Three hosts of grape (Vitis vinifera L), Flame seedless, Thompson seedless, Superior” and two resistant rootstocks “Harmony and Freedom” were inoculated with 3000 second stage juveniles per plant. After two months from inoculation for evaluation of nematode reproduction, Flame seedless was highly susceptible to M. incognita, while Thompson seedless and Superior were moderate susceptible. The difference in protein banding patterns among healthy and infected samples proved they were differences in buffer soluble protein extracted from the leaves of five tested cultivars as the response to infection of M. incognita. ISJ-PCR was used to measure the degree of similarity among grape varieties and rootstock and to calculate the genetic distance between these varieties. Polymorphic fragment reveled the difference and establish systemic relationships among the tested varieties may be referred the resistance in the rootstocks.