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Abstract The expansion of the use of safe alternatives such as bio-fertilizers and organic fertilizer programs in fruit seedlings has become part of a system known as clean cultivation because of its many advantages that contribute to improving growth, productivity, quality and production of a safe crop as well as preserving the environment by reducing or avoiding the use of chemical fertilizers, Therefore, this study was conducted to demonstrate the importance of adding biological fertilizer (Gorabac G) in three concentrations (5, 10 and 15 grams. Seedlings-1), liquid organic fertilizer (Compo) in three concentrations (4, 6 and 8 ml-1) and organic fertilizer (Rice residues (in three concentrations (0.5, 1.0 and 1.5 kg. Seedlings-1) in addition to the comparison treatment (without adding any type of fertilizer) in improving the vegetative growth and mineral content of grapefruit seedlings grafted on Sour orange, he biological fertilizer (Gorabac G) used in the study and in particular the concentration was 15 grams. Seedlings-1 had a significant effect in obtaining the highest values for most of the studied traits, as this treatment was significantly superior to the comparison treatment with the traits (leaf content of nitrogen, phosphorus, potassium, iron, chlorophyll, carbohydrates and proteins in addition to the increase in the height and diameter of seedlings), so within similar circumstances the study recommends using This concentration of this vital fertilizer is to get strong, well-growing seedlings.