Spraying peanut plants under greenhouse and field conditions during seasons 2010 and 2011 with any of the following three growth regulators, indole butyric acid (IBA) and gibbrellic acid (GA3) at 25, 50, and 100 ppm and ethylene (Et) at 0.5, 0.1, and 0.2% resulted in significant reduction of peanut pod rot incidence, occurrence of aflatoxigenic fungi (Aspergillus flavus and A. parasiticus) and peanut aflatoxin contaminations. Ethylene at 0.2% followed by IBA at 100 ppm was the most effective treatments and significantly increased the percentage of healthy apparent pods or pod yield while GA3 at 100 ppm give the lest effect during greenhouse and field experiments. Regard to aflatoxigenic fungi and aflatoxin contamination, isolates of A. flavus were more invasive to either pod shells or seeds than A. parasiticus and occurred at high frequency in seeds compared with pod shells. The most effective treatments were Et at 0.2 %, and IBA at 100 ppm which gave the least incidence of A. flavus and A. parasiticus in all pod rot types and in apparent healthy pods; and reduced preharvest aflatoxins contamination. Increasing of Et and IBA concentrations led to increase their effect in reduction of pod rots incidence or occurrence of aflatoxigenic fungi and peanut aflatoxin contaminations. While increasing of GA3 concentrations gave opposite effect.