Effect of Na2HPO4, NaH2PO4, K2HPO4 and KH2PO4, ascorbic, salicylic and citric, lysine and thiamin, indol acetic acid, giberllin, and pix, was tested on juvenile(j2) mortality and reproduction of M. javanica and the growth of sunflower in vitro and greenhouse. In vitro, 1,250, 2,500 and 5,000 ppm, of those compounds and Rugby, a nematicide, showed a positive correlation between concentration and juvenile mortality.  Rugby was the superior with 100% juvenile mortality, followed by citric acid, 92.7%, then ascorbic acid, 78.9%, and pix, 70.5%, while Na2HPO4 and NaH2PO4 gave, 21.2 and 22.6%,j2 mortality respectively, at 5,000 ppm.

 In greenhouse test, higher shoot dry weights were obtained from the treated than the non-treated plants with the highest shoot weight from plants treated with indol acetic acid, 16.67 gm, and the lowest, 10.0 gm, and 6.0 gm, Na2HPO4 and the control treatments, respectively.  At 1,000 ppm the highest shoot dry weight, 19.33 gm, from plants treated with indol acetic acid or gibberllin and the lowest, 8.67 gm, was detected with thiamine treated plants.  Root dry weights from most treatments did not differ, significantly, than that from the non-treated plants with highest, 8.0 gm, from indol acetic acid at 1,000 ppm and the lowest, 4.0 gm, from lysine at 1,000 ppm or salicylic acid at 2,000 ppm..  Root weights of the control plants ranged from 4 to 5.67 gm

Most treatments reduced reproduction of M.javanica, where the lowest reproductive factor, Rf = 0, was found with K2HPO4, KH2PO4, citric acid, gibberellin or pix treated plants at 1,000 or 2,000 ppm, while ascorbic acid gave a zero Rf at 2,000 but not at 1,000 ppm. The highest Rf values were from plants treated with thiamine, 1.98, or ascorbic acid, 1.88, at 1,000 ppm, and it was 1.69 from thiamine at 2,000 ppm.  The Rf values from the non-treated plants ranged from 1.94 and 1.78.

Regarding to chemical analyses a positive relationship between concentration of the used chemicals and amounts of sugar and phenolic, but not amino acid contents was observed.  Amounts of total sugars ranged from 0.579 to 4.04 mg/gm while amounts of reducing sugars were from 0.229 to 1.246 mg/gmThe highest amount of total phenols, 18.635 mg/gm was found in plants treated with KH222PO4 at 1,000 ppm while the lowest, 6.63 mg/gm was from plants treated with NaH2PO4 at the same concentration.   Amounts of the total amino acids ranged from 0.008 to 0.386 mg/gm from plants treated with KH2PO4 at 2,000 ppm or pix at 1,000 ppm, respectively.


ا.د./ أشرف السعيد خليل

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ا.د./أشرف السعيد خليل

مدير معهد بحوث أمراض النباتات وأستاذ النيماتولوجى- مركز البحوث الزراعية- جيزة. »


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