التخليق الحيوي لجسيمات الفضة النانوية بواسطة عديد السكاريد لبذور Leucaena leucocephala وخواصها المضادة للسرطان والفطريات وكمادة حافظة لعينة الحليب المركبة
Biosynthesis of Silver Nanoparticles by Polysaccharide of Leucaena leucocephala Seeds and Their Anticancer, Antifungal Properties and as Preservative of Composite Milk Sample
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Mohamed A. Taher ,1 Ebtihal Khojah ,2 Mohamed Samir Darwish ,3Elsherbiny A. Elsherbiny ,4 Asmaa A. Elawady ,3 and Dawood H. Dawood 1
1Agricultural Chemistry Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt2Department of Food Science and Nutrition, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia3Dairy Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt4Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, EgyptCorrespondence should be addressed to Mohamed A. Taher; [email protected] 21 November 2021; Accepted 9 January 2022; Published 25 January 2022Academic Editor: José Agustín Tapia HernándezCopyright © 2022 Mohamed A. Taher et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.The AgNPs were synthesized using water-soluble polysaccharides extracted from the Leucaena leucocephala seeds. The UV-visiblespectrum of the AgNPs showed a sharp absorption peak at 448 nm. The XRD analysis showed four major peaks of the crystallineAgNPs with planes of a face-centered cubic lattice of silver. The EDS spectrum showed a strong signal peak at ~3 keV. TEM andSEM observations showed the spherical shape of AgNPs with no particle agglomeration, and the size ranged from 8 to 20 nm.AgNPs were highly stable at -14.2 mV by zeta potential measurement. AgNPs showed significant anticancer activity against thecell lines of breast cancer, liver carcinoma, and colon carcinoma with the IC50 value of 22.5, 12.3, and 8.9 μg mL-1, respectively.AgNPs at 900 μg mL-1 exhibited considerable antifungal activity against ten fungal pathogens. Water-soluble polysaccharide hasthe ability to synthesize AgNPs keeping strong antitumor, antifungal activities. The AgNPs can slow down spoilage ofcomposite milk samples at different temperatures. In addition, the accuracy of milk-IR-analyses was not affected by differentconcentrations of AgNPs.1. IntroductionNanoparticles are of great research attention as they have inno-vative optoelectronic, magnetic, and physicochemical features,affected by their various shape and size details [1]. Researchersare most interested in the biosynthesis of stable nanoparticlesusing green chemistry routes in which no xenobiotic or toxiccompounds are used for environmental sustainability. Greensynthesis of nanoparticles depends on natural reduction com-ponents commonly found in bacteria, fungi, yeast, actinomy-cetes, and plant extracts [1]. In a few recent years, thepolysaccharide-mediated green synthesis of different metalnanoparticles had a voluminous role in nanobiotechnology[2]. Several examples are available of stable metal nanoparticlesbiosynthesis by polysaccharides which chiefly comprise gold,silver, zinc, and palladium nanoparticles.Among zero-dimensional nanomaterials, silver nanoparti-cles (AgNPs) are one of the worthiest candidates for performantand unconventional applications, with enormous outcomes inpharmaceutical sciences, cosmetics, wound dressings, andanti-infective coatings, antimicrobial textiles, and food packages[3, 4]. The major role of AgNPs in medicine mostly depends ontheir exceptional and wide antimicrobial activity [3, 4]. On theother hand, the utilization of nanosilver for prolonging the shelflife of dairy products is not probable as the minimum inhibitoryconcentration of silver is at least 5 mg L-1 in milk which wasgreater than the WHO-recommended maximum concentrationof AgNPs (0.1 mg L-1) [5]. However, to date, there are no data inHindawiJournal of NanomaterialsVolume 2022, Article ID 7490221, 16 pageshttps://doi.org/10.1155/2022/7490221
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(PDF) Biosynthesis of Silver Nanoparticles by Polysaccharide of Leucaena leucocephala Seeds and Their Anticancer, Antifungal Properties and as Preservative of Composite Milk Sample. Available from: https://www.researchgate.net/publication/358126270_Biosynthesis_of_Silver_Nanoparticles_by_Polysaccharide_of_Leucaena_leucocephala_Seeds_and_Their_Anticancer_Antifungal_Properties_and_as_Preservative_of_Composite_Milk_Sample [accessed Mar 18 2022].
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